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KMID : 0363020080380040639
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Journal of Korean Academy of Periodontology
2008 Volume.38 No. 4 p.639 ~ p.644
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Fluorescent detection of bacteria associated with gingival sulcus epithelium
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Shin Seung-Yun
Kye Seung-Beom
Yang Seung-Min
Lee Sang-Hyun
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Abstract
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Purpose: The aim of this study was to compare the number of live and dead bacteria attached to, or within, the stratified squamous epithelium lining the tissue side of the gingival sulcus.
Materials and Methods: A total of 50 patients was examined and classified into healthy or diseased sites according to inflammatory status of the gingival tissue. The surface of stratified squamous epithelium was removed by gentle scraping of the gingival sulcus with curettes. The cells were processed in the laboratory by density-gradient centrifugation to separate the epithelial cells from the loose bacteria and debris. The LIVE/ Bacterial Viability Kit was applied and the specimens were observed by an epifluorescent microscope and the number of bacteria was counted.
Results: Live and dead bacteria were stained to green and red, irrespectively. Generally, the number of total bacteria in the diseased sites was significantly higher than in the healthy sites. The mean number of detected bacteria in the diseased sites was (red bacteria / green bacteria ), while it was in the healthy sites (red bacteria / green bacteria ). The percentage of red bacteria was in the diseased sites and in the healthy sites.
Conclusion: The total number of bacteria in the diseased sites was significantly higher than that of the healthy sites. The ratio and the number of red bacteria were also significantly higher in the diseased sites.
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KEYWORD
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bacterial adhesion, gingival sulcular epithelium, bacterial viability
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